Stable SWNT/hydrogel Composites Patterned in Glass Microchannels for Gel-Free Separation of Biomolecules.

• Main Authors: Jia-Wei Huang, 黃家偉
• Other Authors: Shu-Hui Chen
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/25469596423974339226

碩士 === 國立成功大學 === 化學系碩博士班 === 94 === Abstract Here we report the fabrication of single walled carbon nanotube (SWNT)/organic polymer composites in glass microchannels for application to the separation of biomolecules. The idea is to create mechanically stable SWNT/hydrogel composites which can allow for the use of gel-free buffers in biomolecular separations. Gel electrophoresis has wide applications in protein/DNA separations. In these separations, the gels are mixed with buffers to form static sieving mediums for separation. When transferred to glass devices such as microchip electrophoresis, the gel format causes series problems including high back pressure due to high viscosity. The soft and brittle construct is easily broken by the flow and the torn fragments may cause channel blockage. We are aiming at developing sieving construct that is stable and can sustain the flow. One way to deal with these problems is to immobilize the gels within the microchannels so that separation can be achieved as the molecules move through the immobilized gels. Since the gels are not mechanically strong enough to withstand continuous liquid flow, combining them with SWNT would give the mechanical strength required. We therefore synthesized composites in which SWNT were covalently linked to acrylic polymers to form nanocomposites. The structure of nanocomposites has high strength and stability. Photopatterning provides a fast and simple way to create a controlled nanocomposite phase in the microchannel. The SWNT/hydrogel composites patterned chip can separate three FITC labeled proteins (casein, BSA, IgG) in a short separation length (about 1cm) in less than 90 s. The separation was performed in 90 % TBE buffer pH 8.3-0.1 % SDS and 10 % acetonitrile.