| Summary: | 碩士 === 國立成功大學 === 化學工程學系碩博士班 === 94 === Creatinine is produced from creatine in muscle and is one of the end products of kidney metabolism. It is usually used as an important index to estimate the kidney function. In this study the polymer with specific recognition ability for creatinine is synthesized via molecular imprinting technique. The preparation method involves the sol-gel process. The inorganic precursor, tetraethoxysilane (TEOS), is mixed with the organic functional monomer, 2-acrylamido-2-methyl-propanesulfonic acid (AMPS), through the hydrolysis-condensation reaction, the so-called organic-inorganic hybrid creatinine imprinted polymer is formed. The binding capacity of the polymer towards creatinine in single creatinine solution is 32.57 ± 2.50 mg creatinine/ g MIP and 9.65 ± 1.47 mg creatinine/g NIP from the non-imprinted polymer. The imprinting factor, defined as the binding capacity from the H-MIP to that of H-NIP, is 3.42 ± 0.37. On the other hand, the selectivity ratios in mixture solution comprised of creatine, N-hydroxysuccinimide, creatinine and 2-pyrro-lidinone were 0.053 ± 0.014, 0.034 ± 0.008, 0.854 ± 0.026 and 0.059 ± 0.004, respectively.
As the feasibility of this hybrid material for specific recognition of creatinine is confirmed, the creatinine-recognition polymer film is fabricated on the Au-electrode and is then analyzed via impedometric transducer. Such an electrode is feasible from the initial investigation, but further comfirmation on the sensing effect and the clinical application still need to investigate.
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