| Summary: | 碩士 === 國立中興大學 === 獸醫公共衛生學研究所 === 94 === Abstract
The objective of this study was to determine the feces bacteriology test salmonella isolation of which excrete by pig, dairy cattle, canine and feline, and environment, vector insect in animal farms. The experiment was conducted in the four seasons in 2005. The samples was collected from Taoyuan county and Hsinchu county where locate in north of Taiwan. Sampling was randomly by the number of 2-6% of total head and was according to the feeding phase in pig and dairy cattle. A total of 4366 samples were collected in the experiment and were isolated 590 strains of salmonella (13.51%) from the collected samples. The percentage of isolate were 10% (279/2895) and 19 % (38/204) in pig and dairy cattle. The prevalence rate were 100 % (33/33) and 75 % (3/4) in in pig farm and dairy cattle farm. The serotype groups were mainly B (40.49 %), C (10.41 %), D (1.37 %), E (42.53 %) and Other serogroup (5.2 %). These five serogroup were determined susceptibility against antimicrobials. The result indicated that single medicine resistance was the highest in Tetracyclin and the second was chloramphenicol. The percentage of antimicrobiols susceptibility were 70 %, 58%, 58% and 53% in traditional medicine, such as tetracyclin, chloramphenicol, ampicillin and trimethoprim-sulfamethoxazole, respectively. It’s demonstrated that the four traditional medicines already lost their value in clinical usage. Although, ciprofloxacin were not use in this regional in pig and dairy cattle farm, but the antimicrobiols susceptibility still keep at 17%. The factor of antimicrobiols susceptibility needs to further study. The antimicrobiols susceptibility and prevalence rate in pig farm depend on feed phase, sample sources, management types, feed types, with or without added antimicrobials, season and temperature were analysis. There were not significantly differences (P>0.05) on antimicrobiols susceptibility according to feed type and pathological tissue. However, there were significantly differences (P<0.05) on antimicrobiols susceptibility according to the factors of sample sources, management types, feed types, with or without added antimicrobials, season and temperature by the univariable analysis of chi-square test. The hazard factors of salmonella prevalence which were depend on the factors and were associated with feeding phase in pig and dairy cattle are can be found by using odds ratio of miltivariable logic. We also can set up the hazard point and protection direction. Vector transmission chain can use PFGE analysing to prove their tree relations of phylogene.
|
|---|
