| Summary: | 碩士 === 國立中興大學 === 園藝學系所 === 94 === The study is about the effect of Ethrel on sprouting and development in the different cormel size of Gladiolus ’ Princess Margaret Rose’ cormlets from anther culture in vivo and in vitro. in order to accelerate the speed of breaking the physiological dormancy of the cormel, recover the growth, shorten the time of culture.
The situation of dormancy of Gladiolus ’ Princess Margaret Rose’ cormlets from anther culture had been changed by the different cormlet size, the dormancy were strong in the small cormlte size, and were weak in the large one. The study result could be shown that the sprouting of the cormlets of Gladiolus ’ Princess Margaret Rose’ were 32% in diameter 1~15 cm were higher than when the sprouting were 18% in diameter 0.6-0.9 cm, and the sprout were 10% in diameter <0.6 cm. To affect the corm in vitro with the various concentration of Ethrel in 0, 2.5, 5,and 10 mM, at the fifth week in 5 mM Ethrel, the sprouting were 80% (the medium corm) and 78%(the large corm), both of them had the higher sprouting than others, and the fresh weight were 792 mg(the medium cormlets) and 1453 mg(the large cormlets), also higher than others. The study result could be shown that when Ethrel in 5 mM could break the physiological dormancy of the cormlets of Gladiolus ’ Princess Margaret Rose’ cormlets from anther culture in vitro, recover the growth, shorten the time of culture efficiently.
The sprouting did not raise in medium and large cormlets when we used the 0, 5, 10 mM concentration of Ethrel in Gladiolus ’ Princess Margaret Rose’ cormlets from anther culture in vivo. The sprouting were lower than 25% in the medium cormlets, and 45% in the large cormlets. And the Ethrel did not work on the germinated cormlets, and relative growth ratio were getting down when the culture time were increased, and it were easily to be observed the appearance of blight on the top of the leaf. The blight could be more serious when in the higher concentration of Ethrel.
The effect of the low temperature storage in the sprouting of the cormlets of Gladiolus ’ Princess Margaret Rose’ cormlets from anther culture in vitro, we could see that at the lower 5℃temperature for one month and took it out for 3 weeks, the medium and large cormlets’ sprout rate were 23% and 28%, but in the 25℃, the medium and large cormsel spouting had no obvious different, and the spout rate became lower. It had shown that the low temperature could not break the physiological dormancy of the cormlets of Gladiolus ’ Princess Margaret Rose’ cormlets from anther culture in vitro efficiently. The result of the experiment had showed that, when Ethrel in 5 mM could break the physiological dormancy of the cormlets of Gladiolus ’ Princess Margaret Rose’ cormlets from anther culture in vitro, recover the growth efficiently, and it were more effective to break the physiological dormancy of the cormlets of Gladiolus ’ Princess Margaret Rose’ cormlets from anther culture in vitro more that in the lower temperature storage, Ethrel in 5 mM it helped to increase the sprouting and shorten the time of culture.
|
|---|
