Somatic Embryogenesis via In Vitro Culture of Leaflet Explants of Roses

• Main Authors: Yung-Sheng Hsu, 徐詠勝
• Other Authors: Tsai-Yih Wang
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/35818175410608798770

碩士 === 國立中興大學 === 園藝學系所 === 94 === For increasing embryogenic calli formation rate and reducing time of somatic embryogenesis therefore,A procedure for plant regeneration leaflets of roses（Rosa hybrida L.）via in vitro culture is described. Two auxins, NAA and picloram alone , at various concentrations（0.25,0.5,1or 2mg/l）were tested for its capacity to induce somatic embryogenesis from in vitro-derived leaflet of rose of five cultivars（Christian Dior,Tiffany,Oklahoma,Laser,White Queen Elizabeth）. Calli could be initiated from in vitro leaflet of five rose cultivars on Murashige and Skoog（MS）medium with NAA or picloram. Although all rose cultivars could form calli but, only ‘Tiffany’could produce embryogenic calli on MS medium including NAA（1or2 mg/l）. the influence of genetype on embryogenic differentiation is remarkable. In auxins experiment, leaflet explants were precultured on MS medium with 1mg/l NAA for four weeks followed by subsculture on MS medium with 2mg/l BA.‘Tiffany’showed the highest amount of regeneration plants. Dark incubation periods could reduce time of rose‘Tiffany’inducing somatic embryogenesis and increasing embryogenic callus formation rate.Culture under light or one, four and five of darkness result in embryogenic calli. Dark incubation period of one could produces the highest embryogenic callus and regeneration plantlet. Rose‘Tiffany’needed light to induce somatic embryogenesis. Under 4500lux, embryogenic calli formation rate was higher than other two light intensity treatments and reducing time of somatic embryogenesis. Diferent positions of leaflet might result in callus production, but only middle of leaflet and edge of leaflet could produce embryogenic callus. Middle of leaflet produced higher embryogenic callus rate than that of edge of leaflet.