| Summary: | 碩士 === 國立中興大學 === 食品暨應用生物科技學系 === 94 === The aim of this study was to evaluate the acute toxicity, subacute toxicity (28 days) and immunomodulatory effects of Chinese herbal novel formulas of A and B.
The results from the acute toxicity study of mice administrated with single oral feeding showed that the values of LD50 (medium lethal dose) of formula A and B were 13.27 and 10.00 g/kg BW, respectively, during the 7 days of experimental period. According to the definition of acute toxicity, formulas of A and B were found to be slightly toxic (LD50: 5-15 g/kg BW). During the experimental period, the administration of formula A and B significantly (P < 0.05) increased the secretions of anti-inflammatory cytokine IL-10 by peritoneal macrophage and splenocyte cultures from BALB/c mice. The results suggest that the Chinese herbal novel formula A and B demonstrated a low toxicity and exhibited an anti-inflammatory potential.
Subacute toxicity study (28 days) revealed that supplementation with different doses of formula A and B did not significantly affect the body weight of female BALB/c mice compared to the control group. Most of the levels of GOT, GPT, BUN and creatinine in the serum did not significantly increase, whereas high dose (1330 mg/kg BW) supplementation of formula A significantly (P < 0.05) decreased the serum GOT level. Medium dose (330 mg/kg BW) supplementation of formula B significantly (P < 0.05) decreased the serum glucose level. The results indicate that supplementations of formula A and B demonstrated no obvious toxic effects on the liver and kidney of female BALB/c mice.
The immunomodulatory effects of Chinese herbal novel formula A and B were evaluated using both the in vitro and in vivo studies. In vitro study showed that the administrations of formula A and B at the concentrations of 50, 250 and 500 μg/mL, inhibited the inflammatory responses of lipopolysaccharide-stimulated peritoneal macrophages. After 28 days of consecutive tube feeding of formula A and B to female BALB/c mice, the medium dose (440 mg/kg BW) supplementation of formula A significantly (P < 0.05) decreased the levels of pro-inflammatory cytokines such as TNF-α, IL-6 and IL-1β, secreted by splenocyte cultures. Supplementation with the high dose (1330 mg/kg BW) of formula A and medium dose (330 mg/kg BW) of formula B significantly (P < 0.05) increased the spontaneous secretion of anti-inflammatory cytokine IL-10 by peritoneal macrophages. In addition, supplementation with high dose (1330 mg/kg BW) of formula A as well as low (70 mg/kg BW) and medium (330 mg/kg BW) doses of formula B significantly (P < 0.05) decreased IL-4 production by phytohemagglutinin- stimulated splenocytes. Supplementation with medium (330 mg/kg BW) and high (1000 mg/kg BW) doses of formula B significantly (P < 0.05) increased the serum IgA levels of female BALB/c mice.
Based on the studies of acute toxicity and 28 days of subacute toxicity, Chinese herbal novel formulas of A and B were recognized as safe. Furthermore, both Chinese herbal novel formulas of A and B exhibited differential anti-inflammatory effects and an immunomodulatory potential.
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