| Summary: | 碩士 === 國立中興大學 === 畜產學系 === 94 === The purpose of this study was to evaluate the efficiency of lactoferrin (LF) on early weaning pig. Salmonella typhimurium incubated with lactoferin hydrolyte (from 20 and 40 mg LF/mL) in vitro. The SEM of the cell membrane morphology, showed that bacterial cells exposed to lactoferrin hydrolyte was damaged, more than LF treated bacteria. The damaged extent of LF hydrolyte treated bacteria increased with the incubation time. Seventy-two crossbred 21-day-old weaning pigs were allocated into four dietary treatments, contained 5% rice bran (A), contained 5% LF transgenic rice bran (B) (within LF 50 ppm), and diet A supplement with 50 ppm and 500 ppm commercial LF product as C and D group, respectively. Each treatment had three replicates of six piglets for the 28-day feeding period. The results showed aerobic bacteria, anaerobic bacteria and coliform counts of caecal contents in group with LF transgenic rice bran and commercial LF supplement groups were lower than A group. Plasmic xylose concentration and IgG levels (p<0.05) in C and D group were significantly higher than A and B groups on 28 test days. The demonstrated that supplemented commercial LF treated groups had higher intestinal absorptive ability and immunity activity. In the intestinal mucosa histology, villus hight:crypt depth ratio of D group was greater than other groups. The average daily gain (ADG) of C and D groups were higher than A and B groups. Other ten crossbred 23-day-old weaning pigs were allocated into control and 1% LF treatment groups. After a two-week acclimation period, the piglet were given an LPS (25μg/kg BW) challenge via i.v. to observation the effect of LF on anti-inflammatory. The result showed that LF can decreased the colony of coliform in ileum, and significantly decreased the colony of aerobic bacteria in cecum. The small intestinal morphology and mucosa DNA content of LF group had a tendency towards higher than the control. In the serum characters, the LF treatment could decrease the plasma NO, GLU, AST, BUN concentrations, but not affect the CREA. In conclusion, lactoferrin (from LF transgenic rice bran and commercial LF) could improve the bacteria population of caecum. However, only commercial lactoferrin increased intestinal the absorbability, plamsic IgG concentration, and the growth performance in early weaned piglet, but not the LF transgenic rice bran. Moreover, commercial LF can increase the small inteatinal mucosa DNA, but reduce the plasma GLU, AST, BUN and NO concentration, and decreased damage of inflammatory response.
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