Electric Field Analysis for the Application of Dielectrophoresis on Cellular Experiments

• Main Authors: Huang Yu-Chun, 黃昱畯
• Other Authors: Shu-Hsing Chen
• Format: Others
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/25386366542734478369

碩士 === 崑山科技大學 === 機械工程研究所 === 94 === Molecular biology not only holds clues for medical treatments but also provide engineers with valuable information for designing miniature systems. Among micro-scale manipulation, dielectrophoresis possesses a unique edge: the ability to reach intracellular components non-invasively. Dielectrophoresis is the movement of particles caused by electrical polarization effects in non-uniform AC electric fields. The force responsible for this motion is also governed by the dielectric properties both of the suspending medium and of the particles, as well as the geometry of the electric field. Potential applications of dielectrophoresis in bioengineering research include cell separation and cellular responses to external stimuli. This paper reports results of theoretical investigations on electrode geometry and frequency-dependent properties of particles. Experimental results of dieletrophoreiss on yeast cells and protoplast are also presented.