Direct analysis of methamphetamine and its metabolites in urine by capillary electrophoresis

• Main Authors: Hui-Ling Cheng, 鄭慧玲
• Other Authors: Mei-Shou Wu
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/21750606736989919010

碩士 === 高雄醫學大學 === 藥學研究所碩士班 === 94 === Methamphetamine (M), amphetamine (A), and p-hydroxymeth- am¬phetamine (H) were determined in urine by cation-selective exhaustive in¬jection and sweeping micellar electrokinetic chromatography (CSEI- sweep-MEKC). A phosphate buffer (80 mM , pH 3) containing 20% acetoni¬trile was filled into an uncoated fused silica capillary (40 cm, 50 μm I.D.), then high conductivity buffer (100 mM phosphate, pH 3; 1 psi for 2.5 min) was followed. Electrokinetic injection (10 kV, 600 s) was used to load samples and to enhance sensitivity. The stacking step and separa¬tion were performed by using phosphate buffer (50 mM, pH 3) con¬taining 20% acetonitrile and 100 mM sodium dodecyl sulfate at -20 kV and 200 nm. Using CSEI-sweep-MEKC, the analytes could be simul- ta¬neously analyzed and have a detection limit down to ppb level. During method validation, calibration plots were linear (r ≥ 0.9974) over a range of 100 – 5000 ng/mL for M, and 100 – 2000 ng/mL for A and H. The lim¬its of detection were 20 ng/mL for M, and 15 ng/mL for A and H (S/N = 3). Our method was applied for analysis of 14 real urine samples. The re¬sults showed good coincidence with immunoassay and GC-MS. This method was feasible for application to detect trace levels of abused drugs in forensic analysis.