| Summary: | 碩士 === 高雄醫學大學 === 藥學研究所碩士在職專班 === 94 === Traditional Chinese medicine used in burn injury was Shiunko ointment , which consist of Angelica radix (當歸), Lithospermum radix (紫草), Sesame oil (麻油) and Wax (黃蠟）. But many disadvantages include denaturalization under room temperature, thick sesame smell, oily sensation, hard to cleaning so that it is inconvenient in clinical use. We tried to modify traditional Shiunko ointment and conquer above shortcomings by nanomicell technology.
We use sesame oil, olive oil and mineral oil to extract Angelica radix and Lithospermum radix to make sesame Shiunko oil、olive Shiunko oil and mineral Shiunko oil in our experiment. By HPLC analysis we found sesame Shiunko oil owns the highest concentration of ferulic acid( the mark substance in Angelica radix). Mineral Shiunko oil owns the highest concentration of Shikonin and β,β-dimethylacrylshikonin( the mark substance in Lithospermum radix). The concentration of β,β-dimethylacrylshikonin in Mineral Shiunko oil also higher than the others 50 times.
Adequate amount of glycerol, phospholipids and water was added into each one of sesame Shiunko oil、olive Shiunko oil and mineral Shiunko oil, homogenized by high pressure homogenizer machine, filtered by minipore membrane( 0.1μm), then nanomicell solution was obtained with the particle size less than 100 nm. The stability test showed Nanomicell was stable and particle size still persist less than 100 nm under 25℃storage within 50 days.
To realize the bactericidal effect, 8 kinds of bacterias ( Methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Enterobacter cloacae, Proteus mirabilis, Acinetobacter baumannii) was added into control group, silver sulfadiazine1% cream, traditional Shiunko ointment, sesame Shiunko nanomicell, olive Shiunko nanomicell and mineral Shiunko nanomicell tryptic soy broth. The result revealed that Shiunko nanomicell owns good bactericidal effect to Pseudomonas aeruginosa.
To compare the wound healing ability and wound contraction prevention ability of external medications for burn wounds, animal models of burn injury was performed with 12 New Zealand Rabbits, we cover the 2o burn wounds by silver sulfadiazine1% cream, traditional Shiunko ointment, sesame Shiunko nanomicell jelly,olive Shiunko nanomicell jelly, mineral Shiunko nanomicell jelly, control group and also score them by wound healing scale. The result reveals Shiunko nanomicell jellys owns better wound healing ability than control group, silver sulfadiazine1% cream and traditional Shiunko ointment. Among the three Shiunko nanomicell jellys, olive Shiunko oil nanomicell jelly and mineral Shiunko oil nanomicell jelly had excellent effect in wound healing ability and they were better than sesame Shiunko nanomicell jelly. Olive Shiunko oil nanomicell jelly owns better wound contraction prevention ability.
Rabbits were sacrificed every 5 days and histomorphologic scale to quantify cutaneous scars after burns was performed by biopsy and H&E stain . We found that mineral Shiunko nanomicell jelly had the best outcome in wound healing effect and contraction prevention effect. All the three Shiunko nanomicell jelly are superior then control group, silver sulfadiazine1% cream and traditional Shiunko ointment.
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