Study of the polymorphism of human leukocyte antigen –DQA1, –DQB1 alleles in Chinese patients with chronic hepatitis C

• Main Authors: Chen-Fen Feng, 馮貞芬
• Other Authors: Bai-Hsiun Chen
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/66649191428020758426

碩士 === 高雄醫學大學 === 醫學研究所碩士班 === 94 === Background and Aim : The main cause of non-A non-B hepatitis is hepatitis C virus （HCV）infection. Most acute hepatitis C infection will lead to chroni several studies of hepatitis C with natural history and the relation of host immunity. HCV is associated with particular HLA types, suggesting a role for HLA genes in disease susceptibility. Of the three classes of HLA genes that affect immune function in this complex, the class II DP, DQ and DR seem to be especially important. The purpose of our study is to investigate HLA-DQA1 and HLA-DQB1 genotypes frequency among chronic HCV patients in Taiwan and also investigate the influence of gender, the response to IFN-α therapy on the association of HLA-DQ an c hepatitis C status. Cirrhosis, even hepatocellular carcinoma, will develop 20-30 years after chronic hepatitis C infection. There are several hyperendemic areas in southern Taiwan. Hepatitis C infection will become the major causes of chronic liver disease in the nearly future. Recently there are d HCV susceptibility. Materials and Methods: We investigated 90 patients with chronic HCV infection (male：female= 41：49, mean age= 52.4 ± 13.2 years, age from 24 to 80 years) and 157 healthy control subjects among Chinese living in southern Taiwan using PCR amplification with sequence-specific primers (PCR-SSP) methods. This method is an accurate and rapid technique for detecting genetic variability with a high degree of resolution. Result: Our study revealed that the frequencies of DQAl*0101 alleles were significantly increased in HCV group than that in the controls (pc<0.001). The protective alleles were DQA1*0103 and DQB1*0303 (pc<0.001, pc<0.001, respectively). There was no significant difference of DQA1, DQB1 frequencies between male and female patients. HLA-DQA1 and DQB1 polymorphism were not related to chronic hepatitis C infection with the response to IFN-α therapy. Conclusion: our result indicated that genetic susceptibility and resistance to chronic HCV were associated with particular HLA-DQ alleles in Taiwan Chinese. Additionally, gender could not influence the association of HLA-DQ with chronic HCV. Besides, HLA-DQA1 and DQB1 polymorphism was not be related to chronic hepatitis C infection with the response to IFN-α therapy.