The impact of antiphospholipid antibodies on endothelial cells —A possible mechanism for thrombosis

• Main Authors: Pei-Pei Chen, 陳佩佩
• Other Authors: Wen-Chan Tsai
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/73853544073191027247

碩士 === 高雄醫學大學 === 醫學研究所碩士班 === 94 === Objective. To verify the possible mechanism for thrombosis of Antiphospholipid Syndrome(APS) and the impact of antiphospholipid antibodies(APL) on endothelial cells. Methods. Human umbilical cord vein cells (HUVECs) were incubated with IgG isolated from 12 patients with APS and 3 aCL hybridoma monoclonal antibodies for 6 hours. Expressions of Thrombomodulin (TM), Tissue Factor (TF) and ATP Diphosphosphohydrolase(ATPDase;　CD39) were analyzed on cell surface by Flowcytometry. Levels of soluble TM, soluble TF, von Willebrand factor(vWF) were measured in cultural supernatant by ELISA. Results. Incubation with IgG from 12 patients of APS and IS6 aCL hybridoma monoclonal antibody resulted in a significant decrease of TM on HUVECs. Incubation with IgG from 3 APS patients and IS4 aCL hybridoma monoclonal antibody resulted in a significant increase of TF on HUVECs. No significant differences were detected in CD39 on HUVECs while being incubated with IgG from 12 APS patients, 3 aCL hybridoma monoclonal antibody and 3 normal serum. Soluble TF increased in cultural supernatant after treatment with IgG from 1 APS patient. Soluble TM decreased in cultural supernatant after treatment with IgG from 12 APS patients and CL1 hybridoma monoclonal antibody. vWF level in cultural supernatant increased after treatment with IgG from 4 APS patients and 3 hybridoma monoclonal antibodies. Conclusion. These findings suggest that IgG from patients with APS and hybridoma monoclonal antibodies may create a hypercoagulable state by interaction with endothelial cells. The activation of ECs by aPL is an important mechanism that may precede thrombus formation in patients with APS.