Studies on the Chemical Constituents and Cytotoxicity from the Roots of Tubocapsicum anomalum

• Main Authors: Jyun-Hong Chen, 陳俊宏
• Other Authors: Yang-Chang Wu
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/65112500601801234916

碩士 === 高雄醫學大學 === 天然藥物研究所碩士班 === 94 === Tubocapsicum anomalum （Franch. & Sav.）Makino belongs to Solanaceae family and mainly distributes in the Southeast Asia. This plant is also one of medicine in Taiwan. In a previous study, 23 components from the whole plant of Tubocapsicum anomalum （Franch. & Sav.）Makino had isolated, and their bioactivity were disclosed. In the current investigation, twenty two compounds were isolated from the roots of this plant, including twenty withanolides：tubocapsenolide A (1), tubocapsanolide A (2), 23-hydroxytubocapsanolide A (3), tubocapsanolide F (4), 20-hydroxytubocapsanolide A (5), 17??-hydroxywithanolide D (6), withanolide D (7), tubocapsenolide D (8), tubocapsanolide B(9), tubocapsanolide G (10), iso-tubocapsanolide G (11), 20-hydroxy tubocapsanolide G (12), tubocapsanolide H (13), 2,3-dihydro- 17??-hydroxywithanolide D (14), 24,25-dihydrowithanicandrin (15), withanolide A (16), 23-hydroxytubocapsenolide F (17), anomanolide A(18), 17-epiacnistin A (19) and anomanolide F (20); two steroids：the mixture of ?nβ-stiosterol（21） and stigamsterol（22）, and the mixture of β-sitosteryl-3-O-β-D-glucoside（23） and stigmasteryl-3-O-β-D-glucoside（24）. Among them, 3~5, 10~15, 17, and 20 are new compounds. Compounds 6, 7, 16, and 19, were found for the first time from the genus of Tubocapsicum. The structures of these compounds were identified by spectroscopic and mass data. The cytotoxicity assay indicated that these withanolides exhibited significant cytotoxicity toward liver cancer cell lines（Hep 3B、Hep G2） 、lung cancer cell line（A549） and breast cancer cell lines（MDA-MB-231、MCF 7）.