| Summary: | 碩士 === 中華醫事學院 === 生物科技研究所 === 95 === Selenium (Se) is the important composition of glutathione peroxidase (GPX). Therefore, selenium perform in the role of antioxidation function. The enzyme monoamine oxidase (MAO-B) plays an important role on aging-related diseases, such as Parkinson’s and Alzheimer’s diseases. Because of the observation that MAO-B activity is increased during ageing, it is regarded as the index of ageing. The purpose of this study was to understand the effects of organic / inorganic selenium supplements on GPX enzyme activity, GPX protein expression, and MAO enzyme activity. Firstly, the effects of selenite and seleno-methionine on RAW 264.7 cells were studied. It demonstrated that both organic / inorganic supplements are able to dose-dependent increase the activity and protein expression of GPX. However, the cell growth curves were analysed by MTT method, and it showed that the Se doses used in this study have no influence on RAW 264.7 cell growth. Therefore, our study indicated that the GPX enzyme activity is activated by inorganic / organic Se in RAW 264.7 cells. Secondary, the effects of selenite and seleno-methionine on C6 cells were studied. The concentration (25ng/mL~ 500ng/mL) was used in the medium of C6 cells. It demonstrated that a dose-dependent decrease on MAO enzyme activity was showed by inorganic / organic Se treatments. However, neither GPX enzyme activity nor GPX protein expression increase was observed by inorganic / organic Se treatments in C6 cells. In addition, we further analyzed the effects of Se-containing mineral water (MS) on GPX and MAO by the cell model methods constructed above. The Se-containing mineral water stock (200X) was diluted to 1X, 5X, 10X, and 20X (equal to 9, 45, 90, and 180 ng/mL selenite equivalent). It exhibited that GPX activity increase in RAW 264.7 cells and MAO activity decrease in C6 cells by MS treatments. However, there are no influences on GPX protein expression in RAW 264.7 cells and C6 cells, and no influence on GPX activity in C6 cells treated by MS.
In conclusion, inorganic / organic Se treatments increase GPX enzyme activity and protein expression in RAW 264.7 cells, and decrease MAO enzyme activity in C6 cells. These results may propose the possible application of selenium supplements for the prevention of ageing, Parkinson’s disease, and depression.
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