Performance Enhancement of Capillary Electrophoresis Chip by Surface modification and Design of Entrance Shape

• Main Authors: Fan Sen Lin, 林凡森
• Other Authors: Show-Chuen Chen
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/05668772709944659310

碩士 === 輔仁大學 === 化學系 === 94 === The advantages of sensitivity, speed, low consumption of sample and reagent, and the ease of integration--when annexing parts--with micro capillary electrophoresis are impressive. For one, it is surprisingly sensitive—at sub- orμM level—not too far below that by fluorescence but certainly superior to UV. However, its separation efficiency is inadequate for routine analysis, and besides its speed still has room for improvement. The name of superchip is coined to characterize a chip with high performances of speed, a few tens of seconds averaged for each sample, and separation efficiency with a peak capacity of five or more. This investigation serves the purpose of scouting the necessary traits for a superchip. The literature described the benefit of extra electric field strength by using an hourglass entrance for the separation channel, which, in turn, accerlerated migration. This investigation probes into the effects of entrance-shape induced field enhancement and diffusion suppression from repeated coatings of HPC, a polyelectrolyte, with the injection of dopamine and catechol. The chip performance can be maximized by shaping the entrance as a sherry wine glass to harness the benefit of field enhancement and by precoatings of 8 ppm HPC to suppress the degree of suppression. The sought-after versatility, sensitivity and speed for a superchip may emerge as more research is in progress.