Nuclear- Localization-Signal-Peptides Conjugated with Quantum Dots to Osteoblast Nucleus Labeling

• Main Authors: Yean-R. Lin, 林彥如
• Other Authors: Walter H. Chang
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/97149749566769065638

碩士 === 中原大學 === 醫學工程研究所 === 94 === The purpose of the study was to conjugate quantum dots with nuclear-localization-signal (NLS) peptide to label osteoblasts’ nuclei. The hydrophobic surface of CdSe/ZnS quantum dots (QDs) was modified to became hydrophilic by Poly(maleic anhydride alt-1-tetradecene). Only QDs with amino groups could be conjugated with NLS Peptide. Hence, the surface with carboxyl group of QDs was modified with amino groups using two different type of poly(ethylene glycol). The result showed that tow kind structure of poly(ethylene glycol) modified QDs (PEG-QDs) did not influence the optical nor the physical characterizations of QDs. The salt stability and cytotoxicity test of QDs and PEG-QDs were investigated. It was found that PEG-QDs were more stable than QDs in with high concentration of NaCl, and had lower cytotoxicity of QDs towards osteoblasts and osteoclasts. The Sulfo-SMCC was used for conjugated PEG-QDs with NLS peptides, and these NLS-PEG-QDs were able to labeled osteoblast nuclei. Successfully, the results of this study could be applied to monitor long-term observation of osteoblast nucleus and cell fusion during osteoclastic formation.