Study on the adsorption behavior of fluorescence protein in the pure and modified clays

• Main Authors: Yun-Zhen Tseng, 曾云珍
• Other Authors: Tsung-Yen Tsai
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/85837605592481546599

碩士 === 中原大學 === 化學研究所 === 94 === In this study, the clay, intercalated by cocamidopropylhydroxysultaine, was used to improve the adsorption of DsRed protein. The enhancement of the adsorption of DsRed protein were influenced by the kind of diluted water, pH condition of modified clay, and the concentration of the protein. The optimized process conditions of the protein adsorption has shown when the protein was intercalated/adsorbed by modified clay, preintercalated with cocamidopropylhydroxysultaine (C-50) in pH=7~8 and RO water solvent. The intercalation/adsorption of the protein was highly enhanced from 0.1083 mg/ml to 0.3448 mg/ml when the purified clay was treated by C-50, compared with the purified clay. The result shows that the protein, DsRed, was successfully intercalated/adsorbed in the gallery of clay. There were evidences made by XRD, TGA and FTIR analysis.