Protein kinase G-dependent plasticity in pelvic nerve to urethra reflex plasticity in anesthetized rats

• Main Authors: Pei-yi, 汪佩怡
• Other Authors: Gin-Den Chen
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/97543715085775335054

碩士 === 中山醫學大學 === 醫學研究所 === 94 === Lower urinary tract function of urine storage and micturition are by bladder, bladder neck, urethra and external urethral sphincter (EUS).The bladder major activity is regulated parasympathetic nervous system. The bladder neck is by hypogastric nerve , furthermore, somatic nervous system and autonomic nerve system modulated the urethra. In recent studies on pelvic-urethral reflex (PUR) activities have shown repetitive/titanic stimulation of thepelvic afferent fibers induced a distinct and long-lasting potentiation in PUR activities and the PUR is essential for the urethra to develop sufficient resistance to maintain urine continence. The cGMP and protein kinase G (PKG) signaling pathway were confirmed that has some effects in certain neural cells, in addition, has demonstrated can mediated smooth muscle relaxation. In experimentally, animals were anesthetized with subcutaneous urethane, intrathecal, and then scissor the abdomen to dissociated and transected the pelvic nerve. Furthermore, it used to recording the external urethral sphincter–electromyogram. Repetitive stimulation (RS, 1 Hz) induced a potentiation in the pelvic nerve to urethra reflex (PUR) activities. This potentiation in pelvic-urethral reflex (PUR) activity induced by repetitive stimulation was abolished by APV (D-2-amino-5- phosphonoraleric acid, i.t. 100 μM, 2–5 μl), autocamtide 2-related inhibitory peptide (AIP), Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME 50 mg/ml),1H- [1, 2, 4] Oxadiazolo [4, 3 - a] quinoxalin (in DMSO: 5 mg/mL). Intrathecal L-arginine (50 mg/ml; 20 μl), protoporphyrin IX and 8-Bromoguanosine 3’, 5’-cyclic monophosphate sodium salt monohydrate (8-bromo-cGMP 100 mg/ml 0.22 mM) induced a potentiation of PUR. All these results demonstrated that protein kinase G pathway may be involved glutamatergic NMDA receptor-mediated potentiation in PUR activities.