| Summary: | 碩士 === 中山醫學大學 === 營養學研究所 === 94 === This study was focused on the biological activities of cranberry juice and its various fractions, and also the effect on antioxidant activity and blood lipid regulation in human body. Three fractions of cranberry juice were used in this study, including n-hexane, ethyl acetate（EA）and high polarity fractions (continuous extraction by the order of n-hexane and EA ). Reducing activity, chelating efficiency on ferrous ion, scavenging activity on hydrogen peroxide, DPPH radical and superoxide anion, and also effect on COX-2 and viability on carcinoma cell were all determined by various fractions of cranberry juice. EA fraction was found the main contributor for biological activities of cranberry juice. To further evaluate the effect of cranberry juice on antioxidation and blood lipid regulation in human body. A randomized, placebo control study was designed. Forty subjects were included in this study. Two weeks of run-in period, 10 weeks of administration. Human study showed that 6 weeks of cranberry juice administration greatly increased the antioxidant status, including higher antioxidant capacity, lower thiobarbituric acid reactive substance (TBARS) value, higher ascorbic acid and total thiol contents in blood than those of placebo. In addition, oxidative lag time of LDL was significantly increased from 50.67 to 60.67 (mins). In conclusion, cranberry juice showed great antioxidation, this was probably a prevention of cardiovascular disease. The main contribution was probably mainly from the phenolics of cranberry juice.
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