The effect of liquid extracts and polyphenol extracts from Hibiscus Sabdariffa Linnaeus on nephropathy and lipid metabolism in STZ-induced diabetic rats

• Main Authors: Yu-Hsin, 陳宇欣
• Other Authors: 李彗禎
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/90456763096390499662

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 94 === The major mortality of diabetes mellitus (DM) was usually caused of the complications, such as the cardiovascular diseases and renal failure. This result could be concluded that under the circumstance of high blood sugar for a long time, abundant free radicals could be produced in diabetes patients; and furthermore, the ability of anti-oxidation could be reduced. The oxidative stress could cause macromolecules oxidative and further damage the organ, such as kidney and cardiovascular system. In this study, streptozotocin (STZ) was administrated to induce SD rats type I DM. After the induction completed, 100mg/kg、400mg/kg Hibiscus sabdariffa extracts (HSE) or 100mg/kg、200mg/kg Hibiscus sabdariffa polyphenols (HPE) were fed by oral tube to observe the effects on DM rats. In serum data, we did not observe the restrain result to the blood glucose. However, either HSE or HPE treated groups, the levels of serum albumin, triglyceride, total cholesterol and low density lipoprotein were improved. In TBARs assay, HSE and HPE also possessed the ability to reduce lipid peroxidation in liver. Additionally, in kidney section, we observed the hydropic change caused of the glucose, it would make the cell damage. When HSE or HPE pretreated, this phenomena was improved. Further, in kidney protein lysate, we found HSE or HPE could protect the kidney via increasing Akt expression to acivate Bad and 14-3-3γ expressions, and regulate the transcription factor NF-κB to promote kidney cell survive. We assumed that via reducing oxidative stress, HSE and HPE possessed the ability to improve the high glucose induced abnormal lipid metabolism and protect kidney damage.