Study of autoantibody against to cyclin-dependent kinase inhibitor-2A (p16) and p90 as tumor marker in human lung cancer by ELISA

• Main Authors: Shian-Yi, 邱顯益
• Other Authors: 陳凌雲
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/20718628022522602932

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 94 === The malignant tumour is a main reason to cause the national death, the incidence of the lung cancer is the first of cancers. Nearly 7,000 people die of the lung cancer every year in Taiwan. The first-selected treatment method of the lung cancer is excising with the operation, and the lung cancer that can succeed in excising is only the early case. So the early diagnosis of the lung cancer has extremely important meanings. The tumour cell surface has many kinds of antigen that can cause human immune response. Some antigen only exists in the tumour cell, is called the tumor-specific antigen, TSA. Some antigen is exists in the tumour cell and also exist in some normal cells with very low quanity too, called the tumor-associated antigen, TAA. These tumor-associated antigen cause more cell immune responses of the human body. Documents are pointed out, the scholar finds in the experiment of SEREX that there are autoantibodies that can distinguish the tumor-associated antigen in the cancer patient''s serum. Detect and examine autoantibodies can be regarded as the tumour mark and used for diagnosing the basis of the cancer. The senior of laboratory use ELISA to screen seven TAA autoantibody (IMP1、Cyclin B1、Ras、CSK、Erbb2、p90 and p16) in 60 lung cancer patient. Found finally, TAA p90 autoantibody had appearance rate of 15.00%, and TAA p16 autoantibody had appearance rate of 21.67%. It seems that the result can become the tumour marker of diagnosing the cancer. In order to enrich result with the credibility, we improve examining the quantity of lung cancer patient and rising to 320 from 60. Besides already existing experiment method, the experiment uses the ammonium sulfate to precipitate serum in order to improve the susceptibility. Use different cutoff value to analyse about the experiment. Result show p90 autoantibody have appearance rate of 37.22%, p16 antibody has appearance rate of 35.33%.Find that precipitates the serum with the sulphuric acid ammonium can really improve the susceptibility screened.Comprehensive result of experiment, p90 and p16 autoantibody among lung cancer patient is high than normal person. In other words, p90, p16 autoantibody might suitable for as one immune marker to diagnose lung cancer.