Protection of extracts from Morinda citrifolia against oxidative damage to human blood cells

• Main Authors: Chjen-cheng Chen, 陳建丞
• Other Authors: Dong-Bor Yeh
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/37389938138898505226

碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 94 === Morinda citrifolia commonly known as Noni, is a plant typically found in the Hawaiian, Tahitian and tropical Asia. The bark, stem, roots, leaves, and fruits have been used traditionally as a folk remedy for many diseases including diabetes, hypertension, and cancers. Based on our previous researches, ethanolic extracts of Noni exhibited higher and variety of antioxidative capacity, which were comparable to other solvents (deionized water, ethyl acetate or supercritical fluid carbon dioxide). Ethanolic extracts of Noni have higher total polyphenol and flavonoid contents and reveal that these compounds play a part in antioxidative activities. In this study, we examined the influences of the ethanolic extracts, n-hexane-, n-butanol-, ethyl acetate-, and H2O-soluble partitioned phases obtained from the brown stem, leaf and fruit of Noni on purified healthy human blood lymphocyte using a single-cell gel electrophoresis assay (comet assay). Results showed that n-hexane partitioned phase from ethanolic extract of Noni’s brown stem (BSE-H) at low concentration of 50 ?慊/mL had significant protection against oxidative stress induced by H2O2, whereas at concentration >100 ?慊/mL caused increased DNA damage. Aqueous partitioned phase from ethanolic extract of Noni’s fruit (FE-A) at concentrations from 50 to 200 ?慊/mL had also significant protection against oxidantive stress induced by H2O2, whereas at high concentration (400 ?慊/mL) caused increased DNA damage. Furthermore, n-butanol and aqueous partitioned phases from ethanolic extracts of Noni’s leaf (LE-B and LE-A), n-butanol partitioned phase from ethanolic extract of Noni’s fruit (FE-B) at concentrations from 25 to 400 ?慊/mL suppressed the DNA strand breakage of human lymphocytes induced by H2O2 , and no pro-oxidant effects would be found. 95% ethanolic extract of Noni’s fruit (FE) and ethyl acetate partitioned phase from ethanolic extract of Noni’s brown stem (BSE-EA) at concentrations from 50 to 400 ?慊/mL could suppress the breakage of DNA strand, and be safe to human lymphocytes. From these findings, we suggest that Noni’s brown stem, leaf and fruit are worth further research in purification and identification of active compounds, and will help determine mechanism of protection or damage by phytochemicals.