| Summary: | 碩士 === 中國醫藥大學 === 醫學研究所碩士班 === 94 === High blood lipid and oxidation of low-density lipoprotein(LDL) have been known to play important roles in atherosclerosis. Therefore, lowering blood lipid or prolonging the lag phase of LDL oxidation have potential therapeutic applications to prevent atherogenesis. C-Phycocyanin (CPC) is one of the major biliproteins of Spirulina platensis, a blue green algae, with anti-inflammatory and radical scavenging properties. Lycopene has been shown to be associated with a decreased risk of chronic diseases such as cancer and cardiovascular disease in several recent studies. Lycopene inhibited the activity of an essential enzyme involved in cholesterol synthesis in an in vitro study and a small clinical study, conferring its hypocholesterolemic effect. The purpose of this study was to investigate the effect of CPC and lycopene on the lipid metabolism of hamster fed with a high cholesterol diet. For an 8-week feeding period, 54 male Golden Syrian hamsters (7 week old) were randomly assigned to seven diet groups , including control, 0.2% cholesterol, lopid, C-phycocyanin（0.25％ and 1.25％） and lycopene（3％ and 9％）.Upon sacrifice, the serum was collected from each animal for measurements of total cholesterol, LDL, HDL, and triglyceride. Our results indicated that lycopene and CPC were effective in lowering cholesterol、low density lipoprotein and triglyceride of serum. Regarding the antioxidative activity, the effect on LDL oxidation of CPC and lycopene was assayed by relative thiobarbituric acid-relative substances（TBARS）assay and diene conjugation. Our results showed that CPC and lycopene were able to decrease MDA production in TBARS assay and delayed the diene conjugation in the Cu2+-mediated oxidation of LDL. We have also measured the activity of antioxidative enzymes in liver, including catalase、superoxide dismutases and glutathione peroxidase. The results indicated that the expression of SOD、catalase and glutathione peroxidase were increased in the CPC group and lycopene group as compared to the cholesterol group.These results suggest that the antioxidative and lipid-lowering of CPC and lycopene might be useful in the prevention of the atherosclerotic formation.
|
|---|
