Prevalence of Human Herpesvirus 8 in Taiwan: a comparison among HIV-infected patients, diabetic patients and general population

• Main Authors: Chun-Pin Chang, 張鈞萍
• Other Authors: 林振文
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/38668737093752993885

碩士 === 中國醫藥大學 === 環境醫學研究所碩士班 === 94 === Human herpesvirus 8(HHV-8) may be the cause agent of Kaposi’s sarcoma, primary effusion lymphomas and multicentric Castleman disease. Seroprevalence of HHV-8 varied from 13% to 65% of HIV-infected patients in worldwide countries. However, seroprevalence and risk factor of HHV-8 in HIV-infected patients remain undefined in Taiwan. The purpose of this study was to detect HHV-8 DNA in HIV-infected patients using ORF26 specific nested polymerase chain reaction (PCR), and to compare seroprevalence of HHV-8 among HIV-infected patients, diabetic patients, and general individuals in Taiwan using recombinant ORF66 and ORFK12 antigen-based enzyme-linked immunosorbent assays (ELISAs) and western blotting. The results showed that prevalence of HHV-8 DNA was 7.39% (13/176) in HIV-infected patients. Moreover, ELISA screening indicated seroprevalence was 61.93% in HIV-infected patients, 36.21% in diabetic patients, and 10% in general population. In addition, western blotting conformed that seroprevalence of HIV-infected patients(41.95%) were higher than those of diabetic patients(26.76%) and general population(9%). A significant decrease of HHV-8 seroprevalence was observed in intravenous drug users compared to sexual contact persons using adjusted logistic regression model( aOR=0.11, 95% CI 0.05–0.28, p<0.0001). Patients with ≧200 CD4+(cells/mm3) or ≧1000 HIV viral load(copies/mm3) showed higher seroprevalance(p=0.54, p=0.53). This study has established rORF66 and rORFK12 antigen-based ELISA and western blotting for serological detection of HHV-8 infection, which may be a novel tool for determining more risk factors in association with HHV-8 infection.