Comparison of Aconitine Contents of Crude Drugs of Aconitum Origin Before and After Processing in Taiwan

• Main Authors: Ying-Chun Liu, 劉盈君
• Other Authors: 張永勳
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/65306378042225779375

碩士 === 中國醫藥大學 === 中國藥學研究所碩士班 === 94 === The Wutou and Fuzi have been used in Traditional Chinese Medicine for a long time. They are all drying roots of the Ranunculaceae family. According to the pharmaceutical and chemical studies, the crude drugs of the Aconitum origin comprise a kind of highly toxic diester-diterpene Aconitum alkaloid, namely aconitine, and it also has marked pharmacological effects. After processing, the toxicity might be decrease and the aconite roots could be used safely for the patients. In the present studies, We investigated the crude drugs of Aconitum origin by high-performance liquid chromatography (HPLC), TLC-scanner and LC MS/MS methods to quantify determine of the content of aconitine. We found that aconitine can be reduced by as much as 93% before and after processing. That means processing is very important in Traditional Chinese medicine, especially aconite roots. According to the PRC Pharmacopeia ( 2005 edition), we processed those unprocessed Chaowu and compared the content of aconitine with other crude drugs which were bought at the Chinese traditional medicine stores. The content of aconitine was not different by HPLC. We also compare the content of aconitine of the HPLC with the LC/MS/MS and TLC-scanner, and there is little difference too. And on the DPPH scavering test, we found that it exhibited good free radical scavenging effects in the assay for DPPH scavenging effect, especially processed Chaowu. It had good scavenging effect with less toxicity. According to the Taiwan Herbal Pharmacopeia, We run five tests, including loss on drying, total ash, acid-insoluable ash, diluted ethanol-soluable extractive and water-soluable extractive of those crude drugs of Aconitum origin and investigated the sulfur residue of the white Fuzi. The black Fuzi were recommended that the loss on drying content must be less than 16.30%, total ash content <12.61%, acid-insoluable ash content <1.94%, diluted ethanol-soluable extract content >9.06% and the water-soluable extract content >9.00%; The Chaowu were recommended that the loss on drying content < 14.51%, total ash content <5.59%, acid-insoluable ash content <1.44%, diluted ethanol-soluable extract content >5.13% and the water-soluable extract content must be more than 6.84% . The sulfur residue of the white Fuzi is 0.0137g/kg, and it is less than 500ppm(0.5 g/kg), that means the white Fuzi is safe for clinical used.