| Summary: | 碩士 === 長榮大學 === 職業安全與衛生研究所 === 94 === Workers in synthetic leather manufacturing plants are exposed to N,N-dimethylformamide (DMF) by inhalation and skin contact. Biological samples are collected to conduct biological monitoring and assess workers’ exposure doses. In the metabolic process of human body, DMF can combine with glutathione to form N-methylcarbamoylglutathione (NMG). NMG and NMF can attach to the hemoglobin of red blood cells to form protein adducts, N-methylcarbamoylated valine-globin (NVH). It has been considered that the quantification of NVH in the hemoglobin of red blood cells can more directly estimate workers’ DMF exposure doses. In this study, animal experiments were conducted to simulate workers’ DMF exposures. Different doses (0.0016X, 0.008X, 0.04X, 0.2X and 1X of LD50) of DMF in aqueous solutions were given by gavage to male Sprague Dawley rats. After 14 days of DMF exposure, blood samples of the rats were collected for analysis. A high-performance liquid chromatograph (HPLC) approach was developed to quantify the amount of hemoglobin adducts (Hb adducts) in the blood samples. The method developed in this study showed that the linear relationship between the amounts of NVH in the blood samples and the exposure doses for the rats was statistically significant (r = 0.34). The limit of detection was 73.8 ppb. The recovery efficiency was about 94.18%. This method was used to analyze the blood samples of workers with different concentrations of DMF exposure. There was no statistically significant linear relationship (r = 0.02) between the amount of NVH in the blood samples and the DMF exposure concentrations. This indicated that more researches are needed for applying this method to quantify human DMF exposure. The recovery efficiency of NVH from blood samples was the critical factor relevant to the stability of this HPLC approach to the quantification of Hb adducts. By means of this fast and accurate approach to measuring the NVH in blood samples, it is possible to obtain more understanding on the mechanism of adverse health effect due to DMF exposure.
|
|---|
