| Summary: | 碩士 === 長庚大學 === 醫學生物技術研究所 === 94 === The ESAT-6 and CFP-10 proteins are potent T-cell antigens and secreted by M. tuberculosis complex. ESAT-6 and CFP-10 were encoded by the regions of difference 1(RD1) gene clusters in M. tuberculosiswhich is absent in all BCG substrains. CFP-10/ESAT-6 fusion proteins have been cloned and expressed and the fusion protein’s polyclonalantibodies were also prepared. We use the new biosensor format wasdeveloped by combining ELISA, rapid immunochromatography andelectrochemical method in this study. To achieve a reagentless biosensor, tuberculosis antibody were immobilized onto the electrode surface and
the same anti-tuberculosis antibody conjugated with the horseradish peroxidase (HRP) to produce electron to mediator. The mediator could generate redox on the electrode surface, then transmit electrons to theelectrochemical biosensor. We can detect that whether the specificantigens of M. tuberculosis exist or not. But the detection limit of thisassay is 100ng/ml, we need to improve its sensitivity.
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