The effects of exercise on nasopharyngeal carcinoma interaction with platelet

• Main Authors: chang chuan ying , 張春英
• Other Authors: WANG JONG SHYAN
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/16196188338771051709

碩士 === 長庚大學 === 復健科學研究所 === 94 === Background: Nasopharyngeal carcinoma cell (NPC) is a common cancer in South China. Tumor cell-induced platelet aggregation (TCIPA) confers a number of advantages to the survival of the tumor cell and in its successful metastasis. Moreover, activated platelet can also promote tumor-induced blood coagulation and matrix degradation though activating tissue factor (TF) and metalloproteinase (MMP), thus augmenting tumor-related thrombosis and metastasis.Previous studies have shown that acute exercise affects platelet activity in an intensity-dependent manner. This study is to investigate how various exercise intensities affect reciprocal modulation between NPC and platelet, including hetero-cell aggregation, coagulation, and matrix degradation. Methods: Thirty sedentary healthy men performed on three occasions moderate-intensity exercise (ME, 60%VO2 max for 40 minutes), severe-intensity exercise (SE, up to VO2 max), and SE preceded by warm-up exercise (WUE, 60%VO2 max for 20 minutes) on a bicycle ergometer. Before and immediately after exercise, TCIPA, expression and activity of TF, MMP 2 and MMP 9, as well as amount of tissue factor pathway inhibitor (TFPI) and tissue inhibitor 1 of MMP (TIMP-1) on co-incubation of NPC and platelet were measured. Result: The results of this study demonstrated that (1) ME suppressed the TCIPA induced by fibrinogen, whereas expressions or activities of TF, TFPI, MMP 2 and 9, and TIMP-1 remained unchanged in response to this exercise; (2) SE enhanced the TCIPA induced by fibrinogen, TF activity and expression, MMP 2 and 9 expression, and TIMP-1 content, as well as reduced TFPI content and MMP 2 and 9 activities; (3) SE preceded by WUE suppressed the TCIPA induced by fibrinogen, and was companied with an attenuating effects to SE-mediated expressions or activities of MMPs/TIMP-1 and TF/TFPI. Conclusion: SE enhances hetero-aggregation and coagulation, as well as suppresses matrix degradation though influencing the binding affinity to fibrinogen and reciprocal modulation among TF/TFPI and MMPs/TIMP, this effect diminished after intervention of WUE. Moreover, the ME alone can also minimize the risks of platelet-NPC interactions. Clinical Relevance: The findings presented here provide a choice of safe and effective exercise strategy in cancer patients for disturbing or preventing tumor-related metastasis and thrombosis evoked by platelet.