Functional analysis of KIAA1102 in cell migration
碩士 === 長庚大學 === 基礎醫學研究所 === 94 === To identify human genes that might involve in cell migration, we ectopically expressed these genes in border cells. Interestingly, we found that a novel human gene KIAA1102, which possesses a Calponin homology (CH) domain and a LIM domain, may play a role in regul...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2006
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| Online Access: | http://ndltd.ncl.edu.tw/handle/07940777832641629569 |
| Summary: | 碩士 === 長庚大學 === 基礎醫學研究所 === 94 === To identify human genes that might involve in cell migration, we ectopically expressed these genes in border cells. Interestingly, we found that a novel human gene KIAA1102, which possesses a Calponin homology (CH) domain and a LIM domain, may play a role in regulaton of cell migration because its N-terminal truncated form of KIAA1102 reduced migration about 18%～23% in Drosophila border cells. Loss of E-cadherin at cell junction between border cells and loss of actin amount at the cell cortex were apparent when △CH KIAA1102 was ectopically expressed in border cells. We further analyzed the role of this novel gene in MDCK cell migration. Over-expression of this human gene enhanced the migratory ability of MDCK cells in the transwell migration and wound healing assays. Without both CH and LIM domains of this protein, it loses the ability to promote cell migration in MDCK cells. Immunofluorescence staining showed that KIAA1102 is colocalized with actin filaments. Indeed, either CH or LIM domain of KIAA1102 was able to associate with actin independently. Expression of KIAA1102 also resulted in reduction of E-cadherin expression in MDCK cells. A loss of function assay by knockdown of KIAA1102 in Huh7 cells led to reduce migratory ability of the cell. We will apply GST-pull down assay to know which small GTPase pathway it might be involved in regulation cell migration.
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