| Summary: | 碩士 === 國立中正大學 === 化學所 === 94 === Surface-enhanced Raman scattering (SERS) has been widely used to study the interactions and the orientations of molecules adsorbed on substrate. With its high sensitivity, the amount of samples to be used is reduced greatly. So we can apply it to study expensive samples. Such as DNA for reducing the cost.
The main subject of this thesis is studying the interaction between carbazole derivatives and DNA by SERS. The samples include 3,6-Bis-(1-m-
ethyl-4- vinylpyridinium) carbazole diiodide (BMVC), 3,6-bis (1-2-vinyl py-
ridinium) carbazole (2-BMVC), 3,6-bis(1- methyl-3-vinylpyrazinium) carba-
zole (3NBMVC) and double helix DNA, ds(GCGCAATTG CGC)(LD) solu-
tions. There are differences in the SERS spectrums of carbazole derivative before and after adding LD. By molecular computat ion theory we calculate
the vibration mode of the molecules. By comparision of experimental SERS spectra and calculate Raman spectra, we model how carbazole derivatives absorb on silver nanoparticles.
We compare DODCI, the groove binding molecule with DNA, with BMVC. The SERS spectrums of DODCI and DODCI/LD, exhibit apparent
differences. We conclude that carbazole derivatives do not interact with DNA by groove binding.
No fluorescence is detected when 3NBMVC interacts with DNA. Therefore we take the resonance Raman spectrums of 3NBMVC and 3NBMVC/G- quardruplexes. When 3NBMVC interact with G-quardruplexes,
the solution color changes. However there is no difference observed in the resonance Raman spectrums. The fluorescence of hum24, (TTAGGG)4, in Na+ or in K+ solutions is different. This indicates that Na+ and K+ can cause different hum24 structures.
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