Effect of pH on the Electrophoretic Mobility, Zeta Potential and Ion Condensation of Human Brain Microvessel Endothelial Cell and SLN

• Main Authors: Yi-chun Chen, 陳怡君
• Other Authors: Yung-chih Kuo
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/22435855649986907281

碩士 === 國立中正大學 === 化學工程所 === 94 === The purpose of this study was to investigate the electrophoretic mobility and zeta potential of human brain microvessel endothelial cell (HBMEC) and solid lipid nanoparticle (SLN) with different cacao butter content, stirring rate, stored time and HBMEC coculture with SLN under the influence of pH value. The surface potential of SLN and HBMEC was estimated from the notion of ion condensation of Levin’s theory and Perturbation method. The result revealed that, the absolute value of the zeta potential, electrophoretic mobility, surface charge density and surface potential increased with an increace in pH value. For the same pH value, the more surface charge of biocolloidal particle the more absolute value of the zeta potential, electrophoretic mobility, surface charge density and surface potential. For the discrepancy between surface potential and zeta pontial (Dsz) of HBMEC and SLN, we can find the maximum of Dsz at pH=7 and decreased with increased and decreased pH value.