An association between carotid atherosclerosis and genetic plymorphisms of HO-1 gene promoter, eNOS G894T among residents in an arsenic-endemic area

• Main Authors: Wen-Ling Huang, 黃文玲
• Other Authors: Hung-Yi Chiou
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/17744860885659156953

碩士 === 臺北醫學大學 === 公共衛生學系 === 93 === Carotid atherosclerosis is known as an inflammatory disease and a subclinical syndrome of cerebrovascular, cardiovascular and peripheral vascular disease. The association between arsenic and vascular disease has been proved by many epidemiological studies. Heme oxygenase-1 (HO-1) seems to be a novel protective factor with potent anti-inflammatory and antioxidant effects. Many studies have been carried out to determine the relevance between DNA variants in the eNOS gene and vascular diseases. The aim of the study is to investigate the association between carotid atherosclerosis and genetic polymorphisms of HO-1 gene promoter, eNOS G894T among residents in Lanyan Basin which was a newly confirmed arsenic-endemic area. Residents age≧40 years old were recruited as study population. Among them, a total of 525 study subjects inculing 259 carted atherosclerosis cases and 266 controls were recruited. Each one was examined his/her carotid atherosclerosis by Duplex ultrasonography. Genetic polymorphisms of HO-1 gene promoter, eNOS G894T were detected by PCR, STRP and RFLP. Logistic regression analysis was used to estimate multivariate-adjusted odds ratios and 95% confidence intervals for various risk factors of carotid atherosclerosis. For studying subjects who drank well water with arsenic concentration higher than 50μg/L, and also with HO-1 genotype of [S(GT)n<27] [L(GT)n≧27] and LL have age-sex-adjusted OR of 4.1 and 4.5, respectively, compared with referent group who drank well water with arsenic concentration lower and equal to 50μg/L, and also with SS genotype of HO-1. In addition, after adjustment for age, sex, total choletro, triglyceride, significant increase odds ratios of 4.4 and 4.2 mere observed among study subjects who drank well water contained arsenic level greater than 50μg/L and also with LL and SL genotype of HO-1, respectively. No significant association between G894T polymorphism in exon 7 of the eNOS gene and carotid atherosclerosis was found. These findings might imply that arsenic exposure played more important role on carotid atherosclerosis risk than genetic effects.