Active components isolation and their antimutagenic activities from Solid-state cultivated Taiwanofungus camphoratus via hydroxy radical scavenging effect

• Main Authors: Shu-Chuan Chien, 簡淑娟
• Other Authors: Ching-Lin Hong
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/71548468355533017945

碩士 === 臺北醫學大學 === 公共衛生學系 === 93 === Abstract Taiwanofugus camphoratus is an unique fungal species in Taiwan which is rare and expensive. It has been used as a functional food for drug intoxication, hepatoprotection and anti-tumor. This study used the fruiting body of T. camphoratus obtained from solid stage to further explore its anti-tumor mechanism. Fruiting body was extracted by ethanol and cold water. Ethanol extracts were further partitioned using n-Hexane, ethyl acetate, and methanol fraction. Cold water extracts were eluted through dialysis membrane (M.W. 3000) and precipitated in ethanol which were then used to analyze the bioactivation of T. camphoratus. This study also use the Ames test to evaluate the mutagenesis of T. camphorates. The Salmonella typhimurium TA98, and TA100 mutant strains were used. Either alone or in the presence of S9 mix, T. camphoratus extracts were not mutagenic. The genotoxicity and antimutagenic effects of T. camphoratus extracts were examined using S. typhimurium strain TA102. Our results showed that T. camphoratus extracts were non-genotoxic as well as antimutagenic for TA102, which were pretreated with H2O2-generated ROS in the Ames test. The H2O2 scavenging effect of the T. camphoratus ethanol extracts was highest is the methanol layer, followed by Ethyl acetate layer and n-Hexane layer. Therefore we further isolated three fractions seriesly from the methanol layer by Medium Pressure Liquid Chromatography. Through the antimutgenic assay, Fraction 3 had the highest antimutagenic ability (SR50：0.005mg/ml). In summary, we found that T. camphoratus is antigenotoxic and antimutagenic, which also has the ability of desmutation and antimutation to scavenge free radicals.