Development of Fast Shapable Smart Scaffold Applied in Craniofacial Surgery

• Main Authors: Hsien-Kun Lee, 李憲坤
• Other Authors: Sheng-Yang Lee
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/27332467844683984509

碩士 === 臺北醫學大學 === 口腔科學研究所 === 93 === Craniomaxillofacial surgery or accidental injury frequently results in large bony defects requires the bone grafts to enhance the bone healing. Synthetic bone substitutes such as hydroxyapatite, tricalcium phosphate and calcium sulfate have shortcomings in the sense that they have prolonged working and setting time, poor strain, shear strength and not easily shapeable. Therefore, the purpose of this study is to develop a novel, convenient and easily shapeable bone graft that is composed of poly-lactic acid (PLA) and polycaprolactone (PCL) as the matrix and calcium sulfate as the filler. The principle behind is to utilize the material’s distinct characteristic of fast absorption within the body resulting in the formation of a scaffold with interconnective pores. The optimum ratio of the composition with the best mechanical properties as shown in the result is 18% PLA: 42% PCL: 40% CaSO4. The compressive strength, three point bending strength and flexible modulus were 38.2±1.2 MPa, 67.5±7.2MPa and 7.0±0.7 GPa respectively while the shaping temperature was 50±2 ˚C, the hardening time did not exceed 6 minutes. Cytotoxicity test revealed no mutation or apoptosis present. In addition, good cell attachment ability was also observed. MTT test indicated that cell viability observed in the experimental group was very good. After immersion in phosphate-buffered solution (PBS), 100-300µm sized porosities were observed under scanning electron microscope (SEM) and have potential to be a novel bone graft.