Cyclic RGD-Induced Cell Death in Human Breast Carcinoma (MCF-7) Cells: The Influence of Caspase Pathway

• Main Authors: Tsui-Chin Huang, 黃翠琴
• Other Authors: 陳灝平
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/pz2t5r

碩士 === 國立臺北科技大學 === 生物科技研究所 === 93 === Synthetic peptides containing the arginine-glycine- aspartate (RGD) motif have been used extensively as inhibitors of integrin-ligand interactions in studies of cell adhesion, migration,growth and differentiation, because the RGD motif is an integrin-recognition motif found in many ligands. we designed a cyclic-RGD peptide (Tpa-RGDWPC, cyclic-RGD) with rigid skeleton to bind closely to its acceptor. The cyclic-RGD was obtained by solid phase peptide synthesis method using Rink amide resin via a strategy .we previously reported that cyclic RGD caused cell death in MCF-7 cell line.Apoptosis was observed by 4'', 6-diamidine-2`-phenylindole dihydrochloride (DAPI) staining for morphological changes for biochemical alterations. On the basis of cDNA microarray data, we suggest that the caspase 8 and FADD-like apoptosis regulator, the caspase 9 and inhibitor of apoptosis protein formed the positive and negative feedback control system. These results indicate that cyclic-RGD peptide can induce apoptosis by caspase pathway. The mRNA expression levels of all caspases in MCF-7 treated with cyclic RGD were analyzed and confirmed by real-time quantitative PCR technique.