Studies on Antimicrobial Substance of Endemic Endophytic Fungi

• Main Authors: Shih-Wei Lin, 林世偉
• Other Authors: Shu-Miaw Chaw
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/13828127482003212055

碩士 === 東海大學 === 食品科學系 === 93 === Abstract Endophytes, microorganisms that reside in the tissues of living plants, are relatively unstudied and potential sources of novel natural products for exploitation in medicine, agriculture, and industry. The objective of this study was to screen endemic endophytic fungi which could produce antibacterial substance. Nine strains showed obviously antibacterial activities. Staphyloccus aureus, Bacillus cereus, Salmonella typhimurium and E.coli were used as test organisms. Strain SM-C013 can produce promising antibacterial substance. The strain was identified to be Pestalosphaeria sp. of ascomycota by molecular biological method. Ethanol is the best extraction solvent of SM-C013 antibacterial substance. The inhibiton zone (0.34 cm) of the ethanol extracts was equal to 0.02 µg/ml tetracycline or 5.14 µg/ml ampicillin. The growth of Aspergillus niger, Rhizopus oryzae, Penicillium griseofulvum, Botrytis cinera, and Mucor mucedo were also inhibited by the ethanol extracts. The molecular weight of antibacterial substance is not more than 500 Da.