An association study on genotypes of HLA-A, -B, and -DRB1 and responsiveness to hepatitis B vaccine in Han Chinese and Amis senior high school students in Hualien

• Main Authors: Huei-Wen Chang Liao, 張廖惠文
• Other Authors: Li-yu Wang
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/75574605025292313925

碩士 === 慈濟大學 === 原住民健康研究所 === 93 === Taiwan is an endemic area of hepatitis B virus (HBV) infection. HBV infection can cause acute and chronic hepatitis, cirrhosis, and hepatocellular carcinoma. To control hepatitis B (HB), a mass vaccination program was launched in July 1984. From the results of a series seroepidemiological surveys conducted in Taipei City, this program significantly reduced HBsAg carrier rate of children aged 2-6 years. However, approximately 5-10% of vaccinees were poor or no response to hepatitis B (HB) vaccine. The human leukocyte antigen (HLA) system plays a fundamental role in the homeostasis of immune response to infectious pathogens. We recruited 704 adolescents who had voluntarily participated health examination at entrance in 2003. Genotypes of HLA-A, -B and -DRB1 were determined by sequence-specific oligonucleotide probe hybridization (SSOPH). The relationships between responsiveness to HB vaccine and either genotypes or subtypes of HLA-A, -B and -DRB1 were assessed in Han Chinese (N=350) and Amis (N=147) adolescents separately. We found that HLA-A11, -B40, and -DRB1*04 were the most frequent types in Han Chinese adolescents and HLA-A24, -B40, and -DRB1*04 were the most frequent types in Amis adolescents. In Han Chinese adolescents, the presences of HLA-A*02, and -DRB1*0803 were significantly associated with reduced risk of non-responsiveness to HB vaccine. Sex-adjusted odds ratio (OR) were 0.47 (95% confidence interval [CI], 0.25-0.91; p=0.02) and 0.13 (95% CI, 0.03-0.58; p=0.007), respectively. However, the presences of HLA-B*13 was significantly associated with elevated risk (OR=2.49; 95% CI, 1.03-6.04; p=0.04). In Amis adolescents, OR of non-responsiveness to HB vaccine for the presence of HLA-B*5601 was 4.52 (95% CI, 1.48-13.77; p=0.008). These results indicate that HLA system also plays a significant role in the responsiveness to HB vaccine and the responsiveness to HB vaccine is determined by different HLA-types/subtypes in different ethnic groups.