The Feasibility Study of Scaffold Fabrication in the Cells Incubation Environment for Tissue Engineering Using Rapid Prototyping Technology

• Main Authors: Kuen-Tzan Li, 黎坤瓚
• Other Authors: Jeng-Ywan Jeng
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/88498644095122986215

碩士 === 國立臺灣科技大學 === 機械工程系 === 93 === This research combines the concept of rapid prototype and bioreactor for making scaffold in suitable for the environment that the cell surviving. The purpose lies in expecting to be able to combine the making of the porous scaffold and cultivation of the cell, can continue putting into medium and seeding into the cell after the completing of the scaffold. With the advantage of the bioreactor, it can increase transmission of the medium and reactant. Therefore, enable cell the fast growth at the scaffold. At the same time, this research uses PCL/PLGA (85/15) two kinds of different biodegradable materials to make porous scaffold by rapid prototype system. The materials were soaked in 37℃ PBS and studied the degradation of the materials in vitro. Explain the speed of degradation of the materials via the terms, such as losses of weight , reducing of the molecular weight. Because PLGA (85/15) material comparatively hydrophilic, cause degradation of PLGA (85/15 ) faster than PCL in speed. In addition make porous scaffold with different kinds of porosity, for test the adhesion of osteoblast cell lines(MG-63) by MTT.