| Summary: | 碩士 === 國立臺灣大學 === 藥學研究所 === 93 === Quantitative analyses of gabapentin capsules and vigabatrin tablets were performed in the present study by capillary electrophoresis. Gabapentin and vigabatrin are new antiepilieptic drugs (AEDs) and absorbance of them are very low. Poor sensitivity was obtained by using direct UV detection without derivatization. Because of that, indirect UV detection was used in this study.
During the process of method development, we investigated the influence of possible parameters on resolution, including selection of visualization agents, concentration and pH value of background electrolyte, and applied voltage. The optimal condition was found to be 5 mM m-hydroxybenzoic acid as visualization agent, which was adjusted to pH 11.0 with sodium hydroxide, 30 KV and γ-aminobutyric acid used as internal standard for quantification. Gabapentin and vigabatrin can be separated well in six minutes.
For gabapentin and vigabatrin, repeatability and reproducibility of migration time (RSD) were 0.93 %, 2.00 % and 0.79 %, 2.18 %, respectively; repeatability and reproducibility of peak height ratio (RSD) were 4.71 %, 5.08 % and 4.78 %, 5.52 %, respectively. Recovery were 101.75 % and 100.90 %, respectively. The linear range of gabapentin and vigabatrin were 20-100 μg/mL and 10-50 μg/mL, respectively. The limit of detection were found to be 5 μg/mL and 3 μg/mL, respectively. The method can be used to assay raw materials and formulation products of gabapentin and vigabatrin in a fast and simple way.
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