Establishing a model using small interfering RNAs to inhibit HPV oncogenes E6 and E7

• Main Authors: Yi-chung Lu, 呂一中
• Other Authors: 陳燕惠
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/51492400409058852031

碩士 === 國立臺灣大學 === 藥學研究所 === 93 === The oncogenes E6 and E7 of high-risk HPVs have been identified as the major cause of cervical cancer, one of the most common cancers in women. Due to the discovery of siRNA, which provided a new approach for specific gene silencing, we are interested in whether siRNA could inhibit E6 and E7 gene expression. Since E6 and E7 are transcribed on one mRNA, siRNAs targeting E7 are assumed to inhibit both E6 and E7 gene expression. We synthesized four E7 specific siRNAs including a sequence identical to that used in M. Jiang’s study, two sequences designed according to A. Reynolds’ rules, and a random sequence. Their efficiencies on gene silencing are compared. We demonstrated that all E7 siRNAs were able to inhibit both E6 and E7 gene expression simultaneously except the random si-673. In addition, MTT assays proved the information of significant reduction of cell viability in the treatment of siRNAs. E7 siRNAs may be applied as a promising candidate in the future for HPV therapy.