Microelectrode Array System for Zebrafish Bio-Potential Signals Measurement

• Main Authors: Jin-Jiang Kao, 高錦江
• Other Authors: Chii-Wann Lin
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/83926854515039024301

碩士 === 國立臺灣大學 === 醫學工程學研究所 === 93 === 　　In this research, we developed Microelectrode Array(MEA) chip and measured platform for zebrafish and zebrafish embryo ECG signals and other bio-potential signals .This platform also used in any MEA chip to measure any bio-potential signals. 　　MEA chip is fabricated by using Micro-Electro Machanical System(MEMS) processes. The substrate of MEA chip is glass, and the conduction-line-layer is used Ti(Titanium), Cr(Chromium), ITO(Indium Tin Oxide), and etc. The Bio-Connect-layer is used Au(Gold), ITO, and Pt(Platinum). Our MEA chips are composed of 64 electrodes arranged in an 8 × 8 matrix. The electrodes area are 20 × 20μm2 and 40 × 40μm2. The interval between the two electrodes measures 120μm and 100μm. The sensing area are 1.02 × 1.02mm2 and 1.04 × 1.04mm2. 　　The measurement platform comprise chip holder, measurement circuits, and LabVIEW software platform. We design 64 number of Measurement circuits. Measurement circuits comprise instrumentation pre-amplifier, 300Hz low pass filter, 0.1Hz high pass filter, 60Hz notch filter, and post-amplifier. Becaure DAQ card is limited number of analog signals channels, we design analog multiplexer. We use LabVIEW to control analog multiplexer to catch signals to computer.