Cell Sorter for Leukocyte and Erythrocyte using Dielectrophoresis

• Main Authors: Hsing-han Tsai, 蔡幸翰
• Other Authors: 胡文聰
• Format: Others
• Language: en_US
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/78035834208379171979

碩士 === 國立臺灣大學 === 應用力學研究所 === 93 === This thesis presents a cell sorter that can be integrated into a lab-on-a-chip device for micro total analysis system. Erythrocytes and leukocytes are separated in a microchannel using dielectrophoresis (DEP). Different types of cells/particles have distinct dielectric properties in a particular medium, thus resulting different DEP force. We use the relationship between DEP and drag force to separate and sort the cells. This study shows that a DEP wall can be induced to block large number of leukocytes while letting the erythrocytes pass. Comparison of maximum flow velocity that DEP force is effective in blocking the leukocyte agrees well between experiment and simulation. Results show a sorting rate of about 800 cells per second is achieved. The sorting device is designed with side channels to draw leukocytes out and approaches the sorting goal. We also obtain the relationship between storage time of blood cell and maximum flow velocity, in order to find the best opportune time for separation. This device not only can sort and separate different cells, but also have ability to lyse cells. Therefore, it is a well-integrated microchip with other devices, such as cell counter, PCR chip, and etc.