Microelectrophoresis analysis with a rapid impulse measurement

• Main Authors: Kung-Chu Ho, 何恭竹
• Other Authors: Chieh-Hsiung Kuan
• Format: Others
• Language: en_US
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/48982426538850517948

碩士 === 國立臺灣大學 === 電子工程學研究所 === 93 === Electrophoresis is a technique has long been used to separate biomolecule fragments with different e/m ratios in accordance with medical diagnosis. But there were some shortcomings have long been existed but still cannot be overcome in traditional electrophoresis. Most of which could be analyzed and be taken innovation in a point of view of nanoelectronic technology. First, the buffer medium used in traditional electrophoresis leads to decrease the biomolecular separation efficiency because of thermal diffusion, convection and perturbation motion. That is why high applied electric field could not suitable for old type electrophoresis. Besides, we also want to exclude some poor properties like time-consuming, low repeatability, and use of dangerous carcinogenic fluorescent dye, etc. In this thesis, we bring up a concept base on semiconductor physics to analysis the movement of DNA macroions under an applied impulse voltage. We can obtain the DNA composition with different molecular weights rapidly with just feeding an impulse with high-frequency instead of really “separating” them. By observing RC transient response of different bases, molecular weights and concentrations, we expect that the response curve under the same measuring condition can be repeated. Then we import Ambipolar equations in semiconductor physics to elucidate DNA movement and distribution under applied impulses. And we got an I-V characteristic after sufficient times for measuring, analyzing current component and the reasons of current difference among different DNA sequences. We preliminary confirmed the novel analysis technique is practicable and repeatable. After sufficient data are being acquired, we decide to establish a theoretical model to analyze biomolecular rapid mechanism, and then making a breakthrough for biomedical diagnosis and identification.