| Summary: | 碩士 === 國立臺灣大學 === 農藝學研究所 === 93 === The size and shape of lemma and palea may limit the developing of rice grains, that could affect the rice yield indirectly. Therefore it is interesting to clone genes related to lemma and palea. In this thesis, six genes from the lemma/palea related gene pool which is established by Liu (2003) were further studied. Those genes are salT gene (salt-induced protein), GA-SPY gene (gibberellin action negative regulator SPY-related protein), U2AF gene (U2 snRNP auxiliary factor, small subunit-related protein), kinesin-like gene, DnaJ-like gene, and EF hand gene. At first, the gene expression in leaves and 1~4 cm inflorescences of normal lemma/palea (SLP/SLP), smaller lemma/palea (SLP/slp), and stunted lemma/palea (slp/slp) was compared by Real Time RT-PCR. The results showed that salT expression is higher in leaf sheath than in leaf lamina, and is especially high in the inflorescence of slp/slp mutant. The Southern blot showed that only one copy of salT gene in rice genome. SalT gene was transferred to TNG67 and SLP/SLP rice plant by Agro-transformation method. Three kinds of constructs were designed for over expression, induced expression, and knock out expression of salT gene. The transgenic rice plants with over expressed of salT gene in TNG67 were lower and no spikelets. However, the plant height and the size of lemma/palea of SLP/SLP transgenic rice plants showed no difference to the non-transgenic rice plants. Therefore, the relationship between salT and the structure of spikelets must be studied in more details. The transgenic rice plants with over expressed salT gene showed higher drought tolerance, but no special performance under salt or cold stress.
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