| Summary: | 碩士 === 國立臺灣大學 === 森林學研究所 === 93 === This study describes the development conditions and the propagation technique by in vitro culture for the Ligustrum japonicum Thunb. The primary results are as follow:
Results of mature seeds culture of Ligustrum japonicum were as follows: mature seeds were first treated with running water for 24 hours, soaked in 1﹪anticeptol solution（Benzel thyonium chloride U.S.P 10﹪(w/v), Allcyl arylpolyther alcohol 10﹪(w/v)）for 20 minutes, then soaked in 70﹪ethanol solution for 5 minutes, then soaked in 5﹪NaOCl（supplemented with 0.1﹪(v/v) Tween 20）and treated with ultrasonic shaker for 30 minutes and the seeds could get the 100﹪non- contamination.
The medium of WPM get the best results to establish sterile plants. Light yellow and soft calli were induced after hypocotyls were cultured on WPM medium containing 0.5 ppm 2,4-D under light containing. Rooting was induced after terminal buds were cultured on WPM medium under light containing. Multiple shoots were induced after terminal buds were cultured on WPM medium containing 0.01 ppm IBA and 1 ppm BA under light containing. Multiple buds when transferred to WPM medium lacking plant growth regulators were promoted to elongate their length of internodes under light intensity of 20±5μmol m-2s-1 light environment. The optimal medium for suspension culture was WPM added with 0.1 ppm 2,4-D and 10g/l sucrose of WPM medium.
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