Simulation of Column Chromatograph by A Dimensional Parcel Model

• Main Authors: Chun-Chieh Lai, 賴俊傑
• Other Authors: 白書禎
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/23574040900717900829

碩士 === 國立臺灣大學 === 海洋研究所 === 93 === A continuous Parcel model has been established by incorporating a recently developed discrete Parcel concept with Fick’s Law. The band broadening of an injected sample is assumed attributing to three major factors namely, the initial state, the retention effect and the dispersion effect. An Excel worksheet has been designed to simulate the sample migration route and peak shapes on both longitudinal and temporal axes. The model was applied to analyze experimental data provided by a research group of National Chiao Tung University. The results of peak restoration were found satisfactory. It was also found that the dispersion coefficient (D) is minimal in a chromatographic column, and that the peak shape is mainly controlled by the distribution ratio k” and a parameter (Δt) representing the efficiency of the column system of interest. The mathematical expression for the height of the theoretical plate derived by the present model is very similar to the well-known van Deemter Equation.