| Summary: | 碩士 === 國立臺灣大學 === 物理研究所 === 93 === Electromagnetic tweezers have been demonstrated to be an effective tool in exerting mechanical stimulus in biological cells. By varying the currents applied to the electromagnetic poles and the distance between the tip and cells, force from the piconewton (pN) to nanonewton (nN) can be generated. However, among the cell-mechanical researches which used electromagnetic tweezers to applied constant stimulus to affect cells, the force generated to the cells is restricted to the order of nN.
In this thesis, we combine a single-pole electromagnetic tweezers with a constant-force feedback system on an inverted microscope. By using the graphical programming, LabVIEW, several hardware were integrated in the constant-force feedback system including the charge-coupled device、the motorized stage and the DC power supply, the pN to nN constant force can be applied to a single cell. In the future, we hope to combine this electromagnetic tweezers system with fluorescence microscopy to study the response and mechanism of the cells after affecting by mechanical stimulus.
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