Studies on the molecular identification and secondary endosymbionts of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae)

• Main Authors: Yung-Chang Hung, 洪永昌
• Other Authors: Chiun-Cheng Ko
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/39148795750056597978

碩士 === 國立臺灣大學 === 昆蟲學研究所 === 93 === Bemisia tabaci is a worldwide serious pest possessing more than 24 biotypes; these biotypes are difficult to distinguish by morphological characters only. In this study, we designed the specific primer sets for each of 6 B. tabaci biotypes (A, B, Q, Na, AN, S). Two primer sets, BaAF/BaAR and BaQF/BaQR, for A and Q biotypes, respectively, were based on the sequences of RAPD specific fragments. Four forward primers, BaBF, BaNaF, BaANF, and BaSF, with a common reverse primer, L2-N-3014, for other 4 biotypes, B, Nauru, AN, and S, respectively, were based on their own COI sequence. The 16S ribosomal DNA (rDNA) of the secondary endosymbiont and Wolbachia-specific gene, wsp (cell surface protein of Wolbachia), were amplified and sequenced. Based on the occurrences of 16S rDNA and wsp by PCR, more than 55% and 38% of examined B. tabaci (202 populations) harbored secondary endosymbiont and Wolbachia, respectively. Molecular phylogenetic analysis of 16S rDNA sequences showed that the secondary endosymbiont was located in the clade of γ-3 subdivision of the Proteobacteria. wsp gene sequences, revealed that the Wolbachia found in B. tabaci populations of Taiwan belongs to the group B and subgroup Con/Rug. The results also showed that the carrying/infection rate of secondary endosymbiont/Wolbachia might be related to the biotype of B. tabaci.