Changes in expression of catecholamine and GABA synthesizing enzyme and CREB-signaling elements in olfactory bulb of mouse treated with amphetamine.

• Main Authors: CHEN,CHEN-SHUN, 陳貞勳
• Other Authors: Yin,Hsiang-Shu
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/61148345036964051224

碩士 === 國立臺灣大學 === 解剖學暨細胞生物學研究所 === 93 === The administration of amphetamine could alter the dopaminergic and GABAergic innervation and induced phosphorylation of the transcription factor CREB in the neocortex and striatum. We hypothesize a role of olfactory system in mechanisms for amphetamine action. In this study, adult male wild type SV129 (WT) and monoamine oxidase B (MAOB) knocked out (KO) mice were intraperitoneally injected with saline or amphetamine, 5mg/kg, twice daily for 3 days. On the 4th day, the mice received one injection and perfused with Bouin’s fixative 4h later, followed by preparation of paraffin brain sections and immunohistochemistry. In the olfactory bulb, amphetamine treatment increased the expression of tyrosine hydroxylase (TH) in the perikarya and processes of the glomerular cell layer (Gl) in WT mice, while down-regulated the pCREB immunoreactivity in the granule cell layer (Gr), and GAD-terminals in Gl of WT and KO mice. Furthermore, the drug treatment up-regulated MEK (MAP or ERK kinase) in mitral cells (Mi) of WT and Gl, Mi and Gr of KO. Our data suggest that in the Gl of amphetamine-treated WT, the increased TH level may be associated with down-regulated GAD, whereas in the amphetamine-treated KO, the down-regulated pCREB probably relates to the decreased GAD expression. It appears that distinct layers of olfactory bulb differentially respond to amphetamine.