Summary: | 碩士 === 國立臺灣海洋大學 === 環境生物與漁業科學學系 === 93 === Abstract
The purpose of this thesis is to investigate the toxic effects of two common pesticides ( insecticide and herbicide) on Japanese Medaka (Oryzias latipes). The micronucleus tests examined after 96 hours exposure of 3 different concentrations(1.0, 2.0, 3.0 ppm) of Methomyl and Butachlor, were studied in order to find out the risk of aquatic ecosystems due to the genotoxicity of pesticides. The results are summarized as follow:
1. The occurrence frequencies of micronucleus were slightly increased with dose- dependent response in methomyl experiments.
2. After exposure in common pesticides, erythrocytes were induced to cellular transformations: spherocytes, anisocytosis, echinocytes, vacuolation and haemolysis.
3. Nuclear abnormalities other than micronuclei were found such as notched nuclei, blebbed nuclei, diffused nuclear amterial and enucleated cells.
Key words: Japanese Medaka (Oryzias latipes), pesticide, herbicide, micronucleus
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